DNA Sequencing and Fragment Analysis @ Waterford Institute of Technology (WIT) PMBRC
| Institution | Waterford Institute of Technology (WIT) PMBRC |
|---|---|
| County | Waterford |
| Technique Category | Nucleic Acid Analysis |
| Technique | DNA Sequencing and Fragment Analysis |
Basic Capabilities
Gene sequencing, comparative sequencing, linkage analysis, STR analysis, SNP detection, discovery and validation, mutation detection
Equipment
| Equipment Make | Applied Biosystems |
|---|---|
| Equipment Model | ABI Prism 310 Genetic Analyzer |
Advantages/Limitations
| Advantage of Technique/Equipment | Eliminates gel preparation, can set up runs in minutes, allows the automation of analyses. Cost per sample is quite cheap. |
|---|---|
| Limitations of Technique/Equipment | Run-time per sample can be 30 mins - 3 hours; slower than more expensive alternatives |
Other Details
| Containment | none |
|---|---|
| Turnaround Time | Days |
| Report | yes |
| Interpretation | yes |
| Data on Analyst | Trained PhD student or Experienced postdoctoral researcher |
| Indicative Price | Veries widely depending on sample type, number and amount of analyses required, approx €40 per sample |
Industry Recommendation
Quality & Systems Supports
Quality Management System in Place
Internal quality management system (SOPs, logbooks)
Good Manufacturing Practice Status
Preventative Maintenance
PM contract in place with Applied Biosystems
Accreditation
N/A
