DNA Sequencing and Fragment Analysis @ Waterford Institute of Technology (WIT) PMBRC

Institution Waterford Institute of Technology (WIT) PMBRC
County Waterford
Technique Category Nucleic Acid Analysis
Technique DNA Sequencing and Fragment Analysis

Basic Capabilities

Gene sequencing, comparative sequencing, linkage analysis, STR analysis, SNP detection, discovery and validation, mutation detection

Equipment

Equipment Make Applied Biosystems
Equipment Model ABI Prism 310 Genetic Analyzer

Advantages/Limitations

Advantage of Technique/Equipment

Eliminates gel preparation, can set up runs in minutes, allows the automation of analyses. Cost per sample is quite cheap.

Limitations of Technique/Equipment Run-time per sample can be 30 mins - 3 hours; slower than more expensive alternatives

Other Details

Containment none
Turnaround Time Days
Report yes
Interpretation yes
Data on Analyst Trained PhD student or Experienced postdoctoral researcher
Indicative Price Veries widely depending on sample type, number and amount of analyses required, approx €40 per sample

Industry Recommendation

Contact

Dr. Lee Coffey

Quality & Systems Supports

Quality Management System in Place

Internal quality management system (SOPs, logbooks)

Good Manufacturing Practice Status

Preventative Maintenance

PM contract in place with Applied Biosystems

Accreditation

N/A

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